Peptide Reconstitution Calculator
Enter your vial size, bacteriostatic water volume, and desired dose to calculate concentration, draw volume, and syringe units.
The amount printed on your vial label.
How many mL of BAC water you're adding to the vial.
How much peptide per dose in your research protocol.
Results
U-100 Syringe
This calculator is provided as a research reference tool. Always verify your calculations independently. VialTalk does not sell compounds and is not responsible for any use of this tool.
How Peptide Reconstitution Works
Lyophilized (freeze-dried) peptides arrive as a powder in a sealed vial. Before use in a research protocol, they must be reconstituted — mixed with bacteriostatic water (BAC water) to create an injectable solution. The amount of water you add determines the concentration of the solution, which in turn determines how much liquid you draw for each dose.
This calculator handles the three key conversions researchers need: peptide amount divided by water volume gives concentration in mg/mL, desired dose divided by concentration gives draw volume in mL, and draw volume multiplied by 100 converts to units on a standard U-100 insulin syringe. It also shows how many total doses a single vial provides.
Frequently Asked Questions
How do I calculate peptide reconstitution concentration?
Divide the total peptide amount (in mg) by the volume of bacteriostatic water added (in mL). For example, a 10 mg vial reconstituted with 2 mL of BAC water gives a concentration of 5 mg/mL.
How many units on an insulin syringe equal 1 mL?
On a standard U-100 insulin syringe, 100 units equals 1 mL. To convert your draw volume in mL to syringe units, multiply by 100. For example, 0.1 mL = 10 units.
What is the difference between mg and mcg?
1 mg (milligram) equals 1,000 mcg (micrograms). Many peptides are dosed in micrograms. For example, a 250 mcg dose is the same as 0.25 mg.
How much bacteriostatic water should I add?
The amount is flexible. Common volumes are 1 mL or 2 mL. More water means a lower concentration (larger draws), less water means higher concentration (smaller, more precise draws). Use this calculator to find the volume that gives you a comfortable draw size for your syringe.
What if my dose exceeds 100 units?
If your calculated dose exceeds 100 units (1 mL), split it across multiple draws. Alternatively, add less BAC water to increase the concentration and reduce the draw volume per dose.